膳食钙和VD对高脂膳食大鼠脂质代谢的影响

EFFECTS OF DIETARY CALCIUM AND VITAMIN D ON LIPIDMETABOLISM IN RATS FED HIGHFAT DIETS

目的：研究膳食钙和ＶＤ对大鼠脂质代谢的影响。方法：以Ｗｉｓｔａｒ雄性大鼠为实验对象建立高脂动物模型，按血清胆固醇水平并参考体重将动物分为五组：Ａ组，０．０８％Ｃａ；Ｂ组，０．５％Ｃａ；Ｃ组，０．５％Ｃａ，同时补充ＶＤ；Ｄ组，１．６％Ｃａ；Ｅ组，１．６％Ｃａ，同时补充ＶＤ。Ｃ、Ｅ组以维生素Ｄ３油溶液灌胃（１５μｇ／ｋｇｂｗ），每周两次。实验持续６５天。结果：实验结束时，Ｄ组血清胆固醇和甘油三酯水平显著低于Ａ、Ｂ组，Ｂ组又显著低于Ａ组。Ｃ组的血脂水平与Ｂ组相比无显著性差异，Ｅ组较Ｄ组显著升高。各组的组织脂质含量与血脂水平平行。Ｄ组的粪脂和粪钙含量显著高于Ａ、Ｂ组，Ｂ组又显著高于Ａ组。Ｃ组与Ｂ组相比，Ｅ组与Ｄ组相比，粪脂和粪钙含量皆显著降低。结论：膳食高钙通过减少肠道脂质的吸收而降低血脂水平和组织中的脂质含量。ＶＤ促进钙的吸收而对脂质代谢产生影响，在０．５％Ｃａ基础上补充一定剂量的ＶＤ对于脂质代谢无不良影响；在１．６％Ｃａ的基础上补充同样剂量的ＶＤ对脂质代谢产生不良的影响。

Abstract Objective: This study was designed to investigate the effects of dietary calcium and vitamin D on lipid metabolism and their roles in development of atherosclerosis（AS） in rats fed highfat diet． Methods: 40 male Wistar rats were divided into five groups:A，008％ calcium; B，05％ calcium; C，05％ calcium plus vitamin D; D，16％ calcium; E， 16％ calcium plus vitamin D． The rats in group C and E received vitamin D3 （15μg／kg body weight， ig） twice a week． The study lasted 65 days． Results: Serum cholesterol and triglyceride decreased significantly with increasing dietary calcium． Fecal excretion of total lipids and calcium increased significantly with increasing dietary calcium． Tissue lipids varied with serum lipids parallelly． Although fecal excretion of total lipids and calcium in group C was significantly lower than in group B， no significent difference in serum lipids existed between two groups． However， the levels of serum lipids in group E were significantly higher than in group D． Conclusion: The data suggest that high calcium intake is hypolipemic by increasing fecal excretion of lipids． Normal calcium intake with moderate amount of vitamin D probably has mild beneficial effects on lipid metabolism． However， high calcium intake with the same amount of VD has adverse effects on lipid metabolism in rats fed highfat diet． Key words: dietary calcium vitamin D lipid metabolism