摘要
目的:研究膳食钙和VD对大鼠脂质代谢的影响。方法:以Wistar雄性大鼠为实验对象建立高脂动物模型,按血清胆固醇水平并参考体重将动物分为五组:A组,0.08%Ca;B组,0.5%Ca;C组,0.5%Ca,同时补充VD;D组,1.6%Ca;E组,1.6%Ca,同时补充VD。C、E组以维生素D3油溶液灌胃(15μg/kgbw),每周两次。实验持续65天。结果:实验结束时,D组血清胆固醇和甘油三酯水平显著低于A、B组,B组又显著低于A组。C组的血脂水平与B组相比无显著性差异,E组较D组显著升高。各组的组织脂质含量与血脂水平平行。D组的粪脂和粪钙含量显著高于A、B组,B组又显著高于A组。C组与B组相比,E组与D组相比,粪脂和粪钙含量皆显著降低。结论:膳食高钙通过减少肠道脂质的吸收而降低血脂水平和组织中的脂质含量。VD促进钙的吸收而对脂质代谢产生影响,在0.5%Ca基础上补充一定剂量的VD对于脂质代谢无不良影响;在1.6%Ca的基础上补充同样剂量的VD对脂质代谢产生不良的影响。
Abstract Objective: This study was designed to investigate the effects of dietary calcium and vitamin D on lipid metabolism and their roles in development of atherosclerosis(AS) in rats fed highfat diet. Methods: 40 male Wistar rats were divided into five groups:A,008% calcium; B,05% calcium; C,05% calcium plus vitamin D; D,16% calcium; E, 16% calcium plus vitamin D. The rats in group C and E received vitamin D3 (15μg/kg body weight, ig) twice a week. The study lasted 65 days. Results: Serum cholesterol and triglyceride decreased significantly with increasing dietary calcium. Fecal excretion of total lipids and calcium increased significantly with increasing dietary calcium. Tissue lipids varied with serum lipids parallelly. Although fecal excretion of total lipids and calcium in group C was significantly lower than in group B, no significent difference in serum lipids existed between two groups. However, the levels of serum lipids in group E were significantly higher than in group D. Conclusion: The data suggest that high calcium intake is hypolipemic by increasing fecal excretion of lipids. Normal calcium intake with moderate amount of vitamin D probably has mild beneficial effects on lipid metabolism. However, high calcium intake with the same amount of VD has adverse effects on lipid metabolism in rats fed highfat diet. Key words: dietary calcium vitamin D lipid metabolism
出处
《营养学报》
CAS
CSCD
北大核心
1999年第2期200-206,共7页
Acta Nutrimenta Sinica