摘要
目的:观察乌司他丁(UTI)对脂多糖(LPS)作用下大鼠腹膜间皮细胞(RPMC)白细胞介素-15(IL-15)、白细胞介素-6(IL-6)表达的影响。方法:行RPMC的原代和传代培养,经鉴定后第3代用于实验。随机分组(1)正常对照组;(2)不同浓度脂多糖组(1、10、100mg/L);(3)不同时间组:10mg/LLPS作用于RPMC3、6、12、24h;(4)10mg/LLPS+乌司他丁组(160、320、640U/ml)作用12h;实时定量PCR法测IL-15mRNA的表达;ELISA法检测细胞培养上清液中IL-15、IL-6的表达。结果:脂多糖可刺激RPMC的IL-15mRNA及蛋白的表达增高且在12h内呈时间剂量依赖性(P<0.05);脂多糖诱导RPMCIL-6的蛋白表达增高,在12h内呈时间剂量依赖性(P<0.05);乌司他丁能显著降低脂多糖所致的腹膜间皮细胞IL-15、IL-6的表达(P<0.05)。结论:脂多糖可上调PMCIL-15、IL-6的表达,乌司他丁可抑制脂多糖所致的IL-15、IL-6过度表达,以预防或延缓腹膜炎的发生与发展。
Objective：To observe the effect of ulinastatin on the expression of Interleukin 15（IL-15）,interleukin 6 （IL-6） on cultured rat Peritoneal mesothelial cells （RPMCs） under Lipopolysaccharide conditions.Methods：RPMC were isolated from rat colic omentum and the thirdly cultured PMC were used in the experiment.cultured HPMCs were divided into normal control group,Lipopolysaccharide group（1、10、100 mg/L）,10 mg/L Lipopolysaccharide group and Lipopolysaccharide plus ulinastatin （160、320、640 U/ml）.IL-15 mRNA was detected by Real-time RT-PCR.IL-15,interleukin 6 （IL-6）was detected by ELISA.Results：Compared with the control group,the expression of IL-15,IL-6 was significantly increased in the groups stimulated by LPS（P0.05）.ulinastatin could significantly decrease Lipopolysaccharide induced IL-15,IL-6 expression in a dose-dependent manner both in protein and gene levels（P0.05）.Conclusion：LPS can up-regulate the expression of IL-15,IL-6 in rat peritoneal mesothelial cell.ulinastatin can reverse these changes and it may have significance in the prevention and treating of peritonitis.
出处
《中国中西医结合肾病杂志》
2010年第10期875-878,共4页
Chinese Journal of Integrated Traditional and Western Nephrology
