永生化人骨髓基质干细胞系的生物学特征

Biological characters of immortalized human bone marrow stromal cell line

[目的]明确永生化人骨髓基质干细胞系MSCxj的生物学特征。[方法]MSCxj细胞在连续体外培养过程中,相差显微镜及透射显微镜观察细胞的形态特征;计算群体倍增时间,测定细胞增殖周期,计算克隆形成率等生长增殖特征并与原代人骨髓基质干细胞BMSCs-2比较。[结果]细胞形态为成纤维细胞样,细胞器发达;群体倍增时间40.8 h,较BMSCs-2的43.82 h短,MSCxj生长增殖活跃;MSCxj克隆形成率10.9%,BMSCs-2为11.5%,具有克隆形成能力。[结论]MSCxj细胞保持了人骨髓基质干细胞的生物学特征。

[Objective]To explicate the biological characters of an immortal human bone marrow stromal cell line MSCxj.[Method]The morphology,growth and proliferation of the cells were observed during continuous culture in vitro and compared with that of human bone marrow stromal cells（BMSCs-2）.[Result]The MSCxj cells showed fibroblast-like feature with rich in organelle.The population doubling time（PDT） was 40.8h,it was shorter than the 43.82h of MSCxj＇s PDT.The rate of clone forming was 10.9%,the BMSC-2 was 11.5%.The MSCxj cell grew and proliferated well and were able to form cell clone in vitro.[Conclusion]The MSCxj remains the biological characters of human bone marrow stromal cells.