瞬时感受器电位香草酸受体1参与低氧性人肺动脉平滑肌细胞系的增殖

Transient potential vanilloid receptor 1 participates chronically hypoxic human pulmonary arterial smooth muscle cells proliferation

目的探讨低氧培养的人肺动脉平滑肌细胞系中瞬时感受器电位香草酸受体1(TRPV1)的表达及功能改变。方法用RT-PCR、Real-time PCR和Western blot检测人肺动脉平滑肌细胞中TRPV1的表达。用细胞计数法检测细胞增殖。结果低氧能明显上调人肺动脉平滑肌细胞中TRPV1通道的mRNA和蛋白的表达,并促进增殖,TRPV1通道阻断剂Capsazepine呈剂量依赖性地抑制增殖。结论 TRPV1通道可能参与或调控低氧所致细胞增殖。

Objective To investigate the expression and functional change of the transient potential vanilloid receptor 1（TRPV1） channels in hypoxia-induced proliferation of human pulmonary artery smooth muscle cells（HPASMCs）.Methods RT-PCR,Real-time PCR and Western blot were used to detect the expression of TRPV1 channels in HPASMCs.Cell number was counted with hemocytometer.Results Hypoxia upregulated gene and protein expression of TRPV1 in HPASMCs.The capability of HPASMCs proliferation was significantly increased under hypoxic condition.Capsazepine（a TRPV1 channel inhibitor） inhibited HPASMCs proliferation in a dose-dependence manner.Conclusion These results suggest that TRPV1 channel may be a critical pathway or mediator in hypoxia-induced proliferation of HPASMCs.