hTPO和hNIS共转染提高人胶质瘤、肺癌及甲状腺癌细胞摄放射性碘能力

Increase of radioiodide uptake by co-transfered the human sodium/iodide symporter and the human thyroperoxidase gene into glioma tumor,lung cancer and thyroid cancer cell lines

目的研究共转染人甲状腺过氧化物酶基因(hTPO)及人钠碘转运体基因(hNIS)后胶质瘤、肺癌及甲状腺癌细胞的摄碘能力变化。方法克隆、重组、包装并扩增纯化得到重组腺病毒(AdTPO),测定病毒滴度。使用脂质体转染法将hNIS基因转染入人胶质瘤、肺癌及甲状腺癌细胞系中,经过G418抗生素筛选获得稳定表达hNIS的细胞系,只转染hNIS基因的细胞系为对照组;应用重组腺病毒将hTPO基因传导入对照组中,共转染hNIS及hTPO基因的细胞系为实验组;未转入hTPO和hNIS基因的原始肿瘤细胞系为空白对照组。然后进行3组细胞系的体外摄125I率及各细胞系的125I外流实验。结果在各肿瘤细胞系中,实验组细胞的摄碘率和有效半衰期较对照组细胞及空白对照组细胞均有所提高,实验组细胞的摄碘率:H460组为59 628±1 281,U251组为7 968±1 261,ARO组为52 971±2 162,FRO组为49 638±1 281,3组间总体具有统计学差异(P<0.01)。结论将hTPO和hNIS基因共转染至肿瘤后能有效提高肿瘤的摄碘能力并延长细胞内碘滞留时间。

Objective To investigate the iodide uptake in glioma tumor,lung cancer and thyroid cancer cell lines after co-transfered the human sodium/iodide symporter and the human thyroperoxidase gene.MethodsThrough cloning,recombination,packaging and amplifying,the recombinant adenosine virus AdTPO was constructed.After purification hNIS gene was transfected into tumor cell lines through liposome,hNIS expressing cell lines selected by G418 antibiotics was used as control group.Using AdTPO,hTPO gene was transducted into control group cell lines,as experimental group.Tumor cell lines without hTPO and hNIS gene were applied as blank control groups.Then,we investigated the 125I uptake assay and 125I influx experiments with glioma tumor,lung cancer and thyroid cancer cell lines.Results 125I uptakes and half life in experimental group were longer than that of control groupscell lines.After co-transfecting hNIS and hTPO gene,the uptake ability of 125I was 59 628±1 281,79 638±1 261,52 971±2 162 and 49 638±1 281 counts/min in H460,U251,ARO and FRO tumor cell lines respectirely.Conclusion The uptake of 125I may increase by co-transfection with hNIS and hTPO genes into human tumor cell lines.