急性胰腺炎患者高迁移率族蛋白B1表达增强

Increased expression of high-mobility group box chromosomal protein 1 in patients with acute pancreatitis

目的探讨高迁移率族蛋白B1(HMGB1)在急性胰腺炎(AP)中的表达及其临床意义。方法选择轻症急性胰腺炎(MAP)患者20例,重症急性胰腺炎无器官功能障碍(SAP-1)患者10例,伴器官功能障碍(SAP-2)患者10例,健康志愿者20例,用RT-PCR检测血液中HMGB1mRNA水平,用ELISA法检测血清中HMGB1、TNF-α和IL-6的浓度,分析血清HMGB1水平与急性胰腺炎病情严重程度的关系。结果 MAP组、SAP-1组、SAP-2组血清中HMGB1的浓度分别为(3.5±0.8)、(5.7±1.6)和(8.3±1.7)ng/mL,明显高于对照组的(1.6±0.4)ng/mL;HMGB1mRNA的相对表达分别为0.56±0.11、0.71±0.07和0.89±0.09,明显高于对照组的0.25±0.04。HMGB1的表达均随病情加重而增加。结论 HMGB1在重症急性胰腺炎的病理过程中起十分重要的作用,并且可能是其发生器官功能障碍的一种关键性的细胞因子。

Objective To investigate the expression of high-mobility group box chromosomal protein 1（HMGB1） in patients with acute pancreatitis（AP） and its clinical significance.Methods Blood HMGB1 mRNA level was measured by RT-PCR and serum HMGB1,TNF-α and IL-6 concentrations by enzyme-linked immunosorbent assay in 20 patients with mild AP（MAP）,in 10 patients of severe acute pancreatitis without organ dysfunction（SAP-1） and in 10 patients of SAP with organ dysfunction（SAP-2）.Furthermore,relationship between their serum HMGB1 levels and severity of AP was analyzed. Results The mean value of serum HMGB1 concentrations in patients with MAP,SAP-1 and SAP-2 were（3.5±0.8）,（5.7±1.6） and（8.3±1.7）ng/mL respectively and were significantly higher than that in healthy subjects（1.6±0.4）ng/mL.Blood HMGB1 mRNA levels in patients with MAP,SAP-1 and SAP-2 were 0.56±0.11,0.71±0.07,0.89±0.09 respectively and were significantly higher than that in healthy subjects 0.25±0.04.Serum HMGB1 levels and HMGB1 mRNA were significantly increased in patientswith AP and were correlated with disease severity.Conclusion These results suggest that HMGB1 may act as a key mediator for inflammation and organ failure in AP.