自组装蛋白质芯片--功能蛋白质组学的新工具

Self-assembling protein microarray—a new tool for functional proteomics

传统的蛋白质芯片制备需要进行繁琐的蛋白质表达与纯化。同时,由于蛋白质活性不稳定,蛋白质芯片不宜长期保存。新一代自组装蛋白质芯片,利用无细胞表达体系和DNA固定技术,能够将蛋白质即时、原位表达并固定在芯片上,有效地解决了传统蛋白质芯片的制备和保存问题。目前自组装蛋白质芯片已初步用于大规模蛋白-蛋白质相互作用的筛选,以及鉴定免疫优势抗原等研究。该文介绍了近年自组装蛋白质芯片技术的进展和应用研究。

Traditional protein microarrays require time-consuming processes of protein production and purification since the protein activities are not stable and the array products could not be stored properly for a long time.Self-assembling protein microarrays utilized cell-free expression system and DNA immobilization technique,which allowed multiple-protein expression simultaneously and in situ immobilization on the surface of arrays.This technology effectively overcomes the problems of traditional protein microarrays,and provides a useful tool for functional proteomics analysis.Currently,self-assembling protein microarrays are primarily used for screening protein-protein interactions,identifying immunodominant antigens and so on.This review focused on the recent advancement and application research on self-assembling protein microarrays.