β2-AR27位点基因多态性对气道高反应性的保护性作用研究

The protective effect of the 27-β2 adrenergic receptor genetic polymorphisms in airway hyperreactivity

目的 探讨β2-AR 27位点基因多态性与哮喘的关系及其对气道高反应性的保护作用.方法 采用多聚酶链反应（PCR）-等位基因扩增法,对149例具有气道高反应性的咳嗽变异型哮喘患者进行β2-AR 27位点基因多态性分析检测,同时追踪2年,观察其发展成典型哮喘的比率,并与90名健康对照者进行比较.结果 （1）中国南方汉族人群β2-AR 27位点基因多态性分布以杂合子所占比例较高（57%）,突变型纯合子比率较小（20%）;（2）哮喘组β2-AR 27位点谷氨酸/谷氨酸基因型频率,明显低于健康对照组（9%VS20%）,OR为0.4（P＜0.05）,95%CI为（0.2～0.9）,谷氨酸等位基因频率与对照组差异无统计学意义（P＞0.05）;（3）β2-AR 27位点基因多态性在典型哮喘组谷氨酸/谷氨酸基因型分布频率明显低于哮喘稳定组（P＜0.05）.结论 中国南方汉族人群中27位点基因多态性与哮喘关联,Glu27可能具有气道保护作用.

Objective To analyze the association between β2-AK 27 locus genetic polymorphisms and asthma, and the protective effect in airway hyperreactivity. Methods The allel polymerase chain reaction were used to determine β2-AR 27 locus genetic polymorphisms in 149 patients with cough variant asthma who have the airway hyperreactivity. To observe these people for two years in order to know the proportion of changed to typical asthma. And compare with 90 people in healthy group. Results （1） The distribution frequency of β2-AR 27 locus genetic polymorphisms is major in heterozygote （57 % ） , and the Glu/Glu homozygote has the least （ 20% ） , （2） There was a significant decrease in the frequency of Glu/Glu genotype in asthmatics compared with healthy group（9% VS 20% ） ,OR = 0.4（P〈0.05） ,95% CI （0.2 ～0. 9） ,but there was no significant difference in the allele frequency of asthmatics compared with healthy group,（3）The frequency of Glu/Glu genotype in severe asthma was lower than stable asthma group（P〈0. 05）. Conclusion These results suggesteded that β2-AR 27 locus genetic polymorphisms is correlated with asthma,and the Glu27 could have the protective effect to the airway.