血浆一氧化氮、一氧化氮合酶和基质金属蛋白酶9在子宫颈癌中的临床意义

Clinical significance of plasma nitric oxide, nitric oxide synthase and matrix metalloproteinase 9 in patients with cervical cancer

目的 探讨血浆一氧化氮（NO）、一氧化氮合酶（NOS）和基质金属蛋白酶（MMP-9）水平在子宫颈癌中的变化及临床意义.方法 经病理活检确诊为宫颈癌患者69例,分别于手术前1d、术后15 d、30 d空腹抽取外周静脉血,健康对照组取空腹静脉血,采用化学比色法和ELISA法分别测定血浆中NO、NOS和MMP-9含量,比较上述指标的变化及相关性.结果 宫颈癌患者血浆中NO、NOS、MMP-9的水平与健康对照组相比明显增高.宫颈癌患者手术前与手术后15 d、30 d NO水平比较,手术后NO、NOS、MMP-9的水平均明显降低,但仍明显高于对照组.宫颈癌患者有淋巴结转移者NO、NOS、MMP-9水平明显高于无淋巴结转移者;相关性分析表明,血清中MMP-9与NO、NOS呈明显正相关性.结论 检测血浆中NO、NOS和MMP-9水平的变化,对宫颈癌的病情判断及临床治疗指导具有参考意义.

Objective To investigate the clinical significance of plasma NO,NOS and MMP-9 variences in patients with cervical cancer. Methods 69 cervical cancer patients were studied. Patients with cervical cancer, 1 day before surgery,after 15 days,30 days of fasting peripheral,and healthy control group with fasting blood,venous blood drawn respectively. Plasma NO, NOS and MMP-9 levels were detected by chemical assay and ELISA assay. Comparing the changes in these indicators and their correlation. Results Compared with healthy control group,plasma NO, NOS,MMP-9 levels were significant higher in cervical cancer patients. Compared to before surgery,the plasma NO, NOS,MMP-9 were significantly lower in surgery after 15 days,30 days,but still significantly higher than normal. Patients with cervical cancer with lymph node metastasis NO, NOS, MMP-9 levels were significantly higher than those without lymph node metastasis,correlation analysis showed that serum MMP-9 and NO,NOS had significant positive correlation. Conclusion Detection of plasma NO, NOS and MMP-9 levels, it was better to evaluate the condition of cervical cancer and serve guidelines of clinical treatment.