乌饭树树叶水溶性黄酮的抑菌作用的研究

Study on antimicrobial activity of soluble flavonoids from vaccinium bracteatum thunb.Leaves

为了拓展栽培乌饭树树叶的有效利用途径。采用二倍稀释法和管碟法对乌饭树树叶粗黄酮提取物进行体外抑菌试验,研究了乌饭树树叶黄酮的抑菌作用并和山梨酸钾的抑菌作用做了比较,并测定了温度、pH值和糖盐对其抑菌效果的影响。结果显示乌饭树树叶粗黄酮对细菌的抑制作用明显,但对霉菌和酵母的抑菌效果不明显。其提取液的抑菌效果强于山梨酸钾的抑菌效果。乌饭树树叶粗黄酮在偏酸性条件下抑菌效果较好,随温度的升高其抑菌效果先增强后减弱。乌饭树树叶粗黄酮类化合物在抑菌作用上和糖盐有较强的协同作用。在相同的时间内,提取液的浓度越高,抑菌率越高;相同浓度的提取液,作用时间越长,抑菌率也就越高。因此,乌饭树树叶有作为药品和天然食品杀菌防腐剂开发的潜力。

In order to expand the effective use of cultivation Vaccinium bracteatum Thunb.,the antibacterial activity of crude flavonoids from Vaccinium bracteatum Thunb.leaves was studied with the double-dilution and pipe-plate methods in vitro,studied the antimicrobial effect of crude flavonoids.The results show that the crude flavonoids have obvious inhibition effects on bacteria,and it had no obvious inhibition effects on mildew and yeast.The antimicrobial effect of the crude flavonoids is a higher than potassium sorbic acid.It was also proved that crude flavonoids can more effective in acidic condition,besides the addition of sugar or salt can improve the antimierobial activity.So Vaccinium bracteatum Thunb.leaves have a development potential as drugs and natural food microbicides with Vaccinium bracteatum Thunb.leaves.In the same action time,the higher the concentration of the extraction,the higher of the inhibition rate is.With the same concentration of extraction,the higher the action time will bring higher inhibition rate.So Vaccinium bracteatum Thunb.leaves have a development potential as drugs and natural food microbicides with Vaccinium bracteatum Thunb.leaves.