RP-HPLC法测定β-榄香烯聚氰基丙烯酸正丁酯纳米粒中主药的含量

Determination of β-elemene in β-elemene-Polybutylcyanoacrylate Nanoparticles by RP-HPLC

目的建立反相高效液相色谱法测定β-榄香烯聚氰基丙烯酸正丁酯纳米粒(β-ELE-PBCA-NP)中主药含量的测定方法。方法色谱柱为DiamonsilTMC18(150mm×4.6mm,5μm),流动相为乙腈-水(90:10),检测波长为210nm。结果β-榄香烯浓度在22.4～179.2μg/ml(r=0.9996)的范围内呈现良好线性关系,平均加样回收率为96.9%,RSD分别为1.7%。结论本方法简便、快速、准确,可用于β-ELE-PBCA-NP的质量控制。

Objective To develop a RP - HPLC method for the simultaneous determination of β - elemene in β - elemene - Polybutylcyanoacrylate nanoparticles. Methods The analysis was performed on DiamonsilTM C18 （ 150mm × 4. 6mm, 5μm ）, The mobile phase was acetonitrile - water （90：10） ,The wavelength of the detector was 210nm. Results 13 -elemene presented a good linear correlation in range of 0. 224 - 1. 792μg （ r = 0. 9996 ）. The average recovery was 96. 9% （ RSD = 1.7% ）. Conclusion The method is simple,rapid and accurate, and suitable for the quality control of β - ELE - PBCA - NP