不同地理来源头花蓼的遗传多样性与没食子酸含量相关性分析

Analysis on Genetic Diversity and Gallic Acid Content of Polygonum capitatum in Different Geographical Provenance

应用ISSR-PCR和HPLC方法研究了头花蓼的遗传多样性与没食子酸之间的关系。ISSR结果显示居群总的遗传变异较大(Ht=0.2746),居群内的遗传变异较小(Hs=0.0804),居群间的遗传分化大于居群内的遗传分化。没食子酸含量经SPSS17.0分析显示,各居群间和居群内个体的没食子酸含量差异较大,居群间没食子酸含量范围在0.1738%～0.3306%,其中云南腾冲县,贵州台江县、纳雍县、余庆县、晴隆和毕节县居群间没食子酸含量差异均达到显著水平。通过对头花蓼48个地理居群的遗传多样性与没食子酸含量的相关分析,表明云南腾冲,贵州台江、毕节市亮岩镇、晴隆、盘县居群的遗传多样性指数、没食子酸含量较高,不仅可以作为人工育种的选育材料,而且对于头花蓼种植适生地区划研究具有很好的参考价值。

ISSR-PCR and HPLC method were used to research the relationship between the genetic diversity and the content of gallic acid in P.capitatum.The results of ISSR showed that there was a bigger genetic variation in the total populations（Ht=0.2746） and a smaller genetic variation in the within populations（Hs=0.0804）.The genetic differentiation among populations was greater than those within populations.It was showed that among the various populations and within populations of individual differences had a greater differentiation in gallic acid content by SPSS17.0 analysis.The content of gallic acid were 0.1738%-0.3306% in populations,and it reached a significant level in Tengchong population in Yunnan province,Taijiang,Nayong,Yuqing Xian,Qinglong and Bijie population in Guizhou province.Through the correlation analysis between genetic diversity and gallic acid in 48 geographic populations,it showed that Tengchong,Yunnan,Jiangsu,Bijie-liangyan,Qinglong and Panxian population in Guizhou province,the genetic diversity index,gallic acid of populations were so higher that they could not only be used as artificial breeding materials,but also have good use values for study on regionalization of P.capitatum.