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沉默FNBP1表达诱导7703细胞形态重塑 被引量：3

Silence of FNBP1 Induces Morphological Remodeling of 7703 Cells
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摘要 FNBP1在真核细胞中广泛表达,能通过诱导细胞膜管状化、内陷或变形及后续膜曲率依赖性肌动蛋白聚合过程,参与细胞内吞和运动;还可参与端粒维持及FasL与溶酶体结合过程的调控。研究发现,在肝癌细胞7703中,利用RNA干扰(RNA interferencetechnology,RNAi)技术使内源FNBP1基因表达沉默以后,7703细胞形态发生重塑,由上皮样转变为树状分枝的纤维状,表明FNBP1在细胞形态维持过程中亦具有不可或缺的潜在作用;通过对已知FNBP1蛋白相互作用情况的分析推断,FNBP1可能是通过对肌动蛋白骨架动态组装过程的分子调控参与7703细胞形态的控制。 FNBP1 is an ubiquitously expressed protein in eukaryotic cells, which was involved in endocytosis and cell movement by inducing tubular membrane invagination or deformation and sequential actin polymerization in dependence on membrane curvature, besides telomere maintenance and lysosomal association of FasL. In the present exploration, it was curiously found that knockdown of the endogenous FNBPI with RNA interference technology （RNAi） triggered the morphological remodeling of 7703 ceils from epithelioid to tree-like branched fibrous shape, which strongly indicated that FNBP1 might also play an important role in morphogenesis. It was inferred that FNBP1 governed the cell shape possibly by molecular regulation of actin cytoskeleton assembly from the known FNBP1 protein-protein interactions.
作者 林明明 张乾英 王蕴红 周宇箭 张军
机构地区 重庆医科大学分子医学及肿瘤学研究中心 重庆医科大学细胞生物学及遗传学教研室
出处 《激光生物学报》 CAS CSCD 2010年第5期669-672,共4页 Acta Laser Biology Sinica
基金 国家自然科学基金项目(20803098) 重庆市教育委员会科学技术研究项目(KJ080301)
关键词 FNBP1 7703细胞 RNA干扰 形态重塑 肌动蛋白 FNBP1 7703 cells RNA interference morphology remodeling actin
分类号 Q245 [生物学—细胞生物学] Q26 [生物学—细胞生物学]
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参考文献16

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同被引文献41

  • 1徐霞,訾惠君,孙一娜,任海云.花粉原生质体极性重建及萌发过程中的微丝骨架列阵[J].科学通报,2004,49(15):1507-1511. 被引量：3
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激光生物学报
2010年 第5期

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