近场扫描光学成像结合量子点标记的纳米技术在细胞生物学中的应用

Combination of Near-field Scanning Optical Imaging and Quantum Dot Labeling Based Nanotechnology for Applications in Cell Biology

近场扫描光学显微镜(NSOM)对传统的光学分辨极限产生了革命性的突破,可在超高光学分辨率下无侵入性和无破坏性地对生物样品进行观测。量子点(QDs)具有极好的光学性能,如荧光寿命长、激发谱宽、生物相容性强、光稳定性好等优点,适合先进的生物成像。NSOM结合QDs标记的纳米技术被应用在细胞生物学中。通过纳米量级NSOM免疫荧光成像(50nm)对特定蛋白分子在细胞表面的动态分布进行可视化研究和数量化分析,阐明了蛋白分子在不同细胞过程中的作用机制。因此,NSOM/QD基成像系统提供了单个蛋白分子最高分辨率的荧光图像,为可视化研究蛋白分子机制的提供了一种强有力的工具。

Near-field scanning optical microscope （NSOM） breaks through conventional optical diffraction limit, which provide ultra-high resolution detection for the biological sample without invasion and injury. Quantum dots （QDs） have fascinating optical properties, including long fluorescence lifetime, wide luminescence spectra, high biological compatibility, and good photostability, making it suitable for advanced biological imaging. Combination of NSOM and QDs labeling based nanotechnology is applied in the cell biology. The dynamic distribution of specific protein molecules on the cell surface has been visually studied and quantitatively analyzed by nanoscale NSOM immunofluorescence imaging （50 nm） , clarifying the mechanism of the protein molecule in different cellular processes. Therefore, NSOM/QD based imaging system has generated the best optical resolution for the immunofluorescenee images of single protein molecule, providing a powerful tool for visually studying the mechanics of the protein molecules.