急性一氧化碳中毒迟发性脑病大鼠模型的建立

Establishment of Rat Model for Delayed Encephalopathy after Acute Carbon Monoxide Poisoning

目的探讨建立适合实验研究需要的急性一氧化碳中毒迟发性脑病大鼠模型。方法 64只雄性SD大鼠,随机分成空气对照组(AC组)和模型组(CO组),每组32只。采用静态吸入式染毒,选取染毒后3、7、14、28d为时相点,应用Morris水迷宫实验检测平均潜伏期等学习记忆能力,Tunel法检测海马回神经元凋亡。结果 CO组大鼠较AC组平均潜伏期延长(F=35.93,P<0.01),海马回神经元凋亡指数增高(P<0.01)。结论本实验成功建立了可行性较高的急性一氧化碳中毒迟发性脑病大鼠模型,为进一步研究其发病机制和防治方法提供比较好的基础。

Objective To establish a rat model for delayed encephalopatby after acute carbon monoxide poisoning. Method 64 male SD rats were randomly divided into two groups ： control group and experimental group, each group with 32 rats. The static inhaled exposure method was used to make the rat poisoning. Movis water maze test was used to measure the learning and memory ability such as the average latency on 3ra ,7th, 14th and 28th day after poisoning. The apoptosis of pyramidal neurons in hippocampal gyrus area was measured by in situ apoptosis detection. Results In Morris water maze test, the average latency in CO group was longer than that in AC group（ F = 35.93, P 〈 0.01 ）. The apoptotic index of hippocampal gyrus neurons in CO group was increased significantly compare with that of AC group （ P 〈 0.01 ）. Conclusion A delayed encephalopathy after acute carbon monoxide poisoning rat model was established. It provided a useful tool for further investiga- ting the pathogenesis and cure methods of the encephalopathy induced by carbon monoxide poisoning.