不同浓度咖啡因对犬精液冷冻效果的影响

Effect of Different Concentration of Caffeine in the Basic Sperm Dilution on Quality of Frozen Canine Sperm

为提高犬冷冻精液的冷冻效果,试验向Tris-卵黄基础稀释液中添加不同浓度的咖啡因;结果表明:当所添加的咖啡因浓度为5.0mmoL/L时,精子活率为0.33±0.04(P<0.01),显著>对照组。添加浓度为12.5mmoL/L时,精子活率仅为0.23±0.04(P<0.01),显著<对照组。浓度为5.0mmoL/L时精子畸形率最低,为0.174±0.036(P<0.01),显著<对照组。但添加咖啡因的各组跟对照组在顶体完整率上并无显著差异。

This study was aimed to improve the frozen-thawing effect of the dog＇s sperm. The experiments include the effect of different concentration of caffeine in the basic sperm dilution on quality of frozen canine sperm. The results showed that when the caffeine concentration got 5.0 mmoL/L,the dogs＇ spermatozoa progressive motility was significantly higher than the control one （0.33±0.04 v.s 0.27±0.03,P0.01）. While the caffeine concentrations was 12.5 mmoL/L,the dogs＇ permatozoa progressive motility was significantly lower than the control one （0.22±0.05 v.s 0.27± 0.03,P 0.01）. In addition,while the caffeine concentrations was 5.0 mmoL/L,the dogs＇ sperm deformity rate was significantly lower than the conrtol one （0.174 ±0.039 v.s 0.203 ±0.036,P 0.01）,but there was no significant discrepancy on acrosome intactness rate between them.