HPLC同时测定肉桂中香豆素、桂皮醇、桂皮酸、桂皮醛的含量

Simultaneous Determination of Cumarin,Cinnamyl Alcohol,Cinnamic Acid and Cinnamaldehyde in Cortex Cinnamomi by HPLC

目的建立反相高效液相色谱法测定肉桂药材中香豆素、桂皮醇、桂皮酸、桂皮醛的含量。方法色谱柱为Hypersil C18（4.6 mm×250 mm,5μm）;流动相：乙腈-0.1%磷酸（28∶72）;流速：1 mL.min-1;测定波长：280,254 nm;柱温：30℃。结果 4种成分分离良好,均有良好的线性范围,分别为香豆素0.035 4~1.768μg（r=1.000）,桂皮醇0.020 2~1.008μg（r=1.000）,桂皮酸0.026 4~1.318μg（r=1.000）,桂皮醛0.113~5.637μg（r=1.000）,平均回收率分别为101.4%（RSD为0.56%）,103.7%（RSD为1.42%）,100.2%（RSD为0.73%）,97.4%（RSD为1.16%）。结论本方法简便、灵敏、准确,适用于肉桂药材的质量评价。

OBJECTIVE To establish a RP-HPLC method for the simultaneous determination of cumarin,cinnamyl alcohol,cinnamic acid and cinnamaldehyde in Cortex Cinnamomi.METHODS The separation was performed on a Hypersil C18 column（4.6 mm×250 mm,5 μm）at 50 ℃.The mobile phase was acetonitrile-0.1% phosphoric acid（28∶72） at a flow rate of 1 mL·min-1.The detection wavelenge were set at 280 nm and 254 nm.RESULTS All of 4 compounds were separated and good linearity was obtained in the rangeths 0.035 4～1.768 μg for cumarin（r=1.000）,0.020 2～1.008 μg for cinnamyl alcohol（r=1.000）,0.026 4～1.318 μg for cinnamic acid of（r=1.000）,0.113～5.637 μg for cinnamaldehyde（r=1.000）.The average recoveries were 101.4%,103.7%,100.2% and 97.4% with RSD 0.56%,1.42%,0.73%,and 1.16%,respectively.CONCLUSION The method is simple,sensitive and accurate,and it can be used for quality eveluation of Cortex Cinnamomi.