核因子-κB在难治性全身型幼年特发性关节炎患儿外周血单核细胞核内表达的意义

Significance of Expression of Nuclear Factor Kappa B in Monocytes of Peripheral Blood from Children with Refractory Systemic-Onset Juvenile Idiopathic Arthritis

目的研究核因子-κB(NF-κB)在难治性全身型幼年特发性关节炎(SOJIA)发病机制中的作用。方法收集难治性SO-JIA患儿(难治性SOJIA组)活动期9例、非难治性SOJIA患儿(非难治性SOJIA组)活动期11例和对照组儿童9例作为研究对象。通过免疫磁珠分离出抗凝血中单核细胞,提取其核蛋白。采用凝胶迁移实验法检测其NF-κB的表达水平;并以ELISA法检测其外周血血清TNF-α的表达水平。结果 1.NF-κB在3组中表达水平差异有统计学意义(F=5.887,P<0.05)。NF-κB在难治性SOJIA组表达最高(35.01±14.78),与非难治性SOJIA组(23.48±11.52)和对照组(17.43±3.62)两两比较差异均有统计学意义(Pa<0.05)。2.TNF-α水平在3组中表达水平差异有统计学意义(F=51.701,P<0.001)。TNF-α在难治性SOJIA组表达最高[(30.38±3.21)ng.L-1],与非难治性SOJIA组[(18.13±3.31)ng.L-1]和对照组[(16.60±2.98)ng.L-1]两两比较差异均有统计学意义(Pa<0.05)。3.SOJIA患儿外周血单核细胞核内NF-κB与外周血血清TNF-α水平呈正相关(r=0.464,P<0.05),但与PLT、ESR、CRP、单核细胞计数等指标无相关性。结论难治性SOJIA患儿体内存在高表达的NF-κB和TNF-α,且这些因子对于维持炎症的持续发展具有重要意义。NF-κB在临床上或许可以作为SOJIA患儿病情严重程度的预测指标之一。

Objective To study the effect of nuclear factor kappa B（NF-κB） in the pathogenesis of refractory systemic-onset juve-nile idiopathic arthritis（SOJIA）.Methods Monocytes had been isolated with immunomagnetic beads from anticoagulated blood from 9 cases of refractory-SOJIA children（refractory-SOJIA group） at active stage,11 cases of non-refractory SOJIA children（non-refractory SOJIA group） at active stage,and 9 cases of control children.And then the expression levels of NF-κB were detected with electrophoretic mobility shift assay after the protein extraction.The levels of TNF-α were measured by enzyme-linked immunosorbent assay（ELISA）.Results 1.There was statistical significance of NF-κB levels in the 3 groups（F=5.887,P〈0.05）.The significant difference was shown among the refractory SOJIA group（35.01±14.78）,non-refractory SOJIA group（23.48±11.52） and the control group（17.43±3.62） in the level of NF-κB（Pa0.05）.2.There was a statistical significance of TNF-α expression in 3 groups（F=51.701,P〈0.001）.Compared with the non-refractory SOJIA group[（18.13±3.31） ng.L^-1 and the control group[（16.60±2.98） ng.L^-1,the refractory SOJIA group[（30.38±3.21） ng.L^-1 was the highest one（Pa0.05）.3.There was a correlation between the levels of NF-κB and TNF-α（r=0.464,P〈0.05）,but it showed no correlation between the level of NF-κB and the number of monocytes,PLT,ESR and CRP.Conclusions There are high levels of NF-κB and TNF-α in the refractory SOJIA children,and these factors are significant in maintaining the sustai-nable development of inflammation.NF-κB may be able to forecast as one of the indicators in clinical severity.