OX40配体单克隆抗体对过敏性紫癜患儿外周血OX40/OX40配体表达的影响

Effect of Anti-OX40 Ligand mAb on Expression of Costimulatory Molecules OX40 and OX40 Ligand on Peripheral Blood in Children with Henoch-Schonlein Purpura

目的探讨共刺激分子OX40/OX40L在过敏性紫癜(HSP)患儿外周血单个核细胞(PBMC)中的表达及其在HSP发病机制及治疗中的作用及意义。方法选取首次住院HSP患儿34例(HSP组),另取健康体检儿童20例作为健康对照组。分别采集外周静脉血5 mL,用密度梯度离心法制备新鲜PBMC,分为对照培养组;植物血凝素(PHA)刺激组;PHA+OX40L单克隆抗体(Anti-OX40LmAb)组。培养48 h后分别抽取RNA,应用反转录-PCR(RT-PCR)从转录水平检测PBMC中OX40 mRNA和OX40L mRNA的表达。结果在对照培养组中,HSP组OX40 mRNA、OX40LmRNA的表达均明显高于健康对照组(Pa<0.01)。加入PHA刺激后,2组OX40 mRNA、OX40LmRNA表达均明显增高(Pa<0.01),且HSP组较健康对照组明显增高(Pa<0.01);加入Anti-OX40LmAb后2组OX40 mRNA、OX40LmRNA表达均明显下降(Pa<0.01)。结论 HSP患儿PBMC共刺激分子OX40和OX40L的mRNA表达异常增高,OX40/OX40L这一对共刺激分子可能参与了HSP的发病及发展,阻断该共刺激通路有望为HSP的治疗提供新思路。

Objective To investigate the expressions of costimulatory molecules OX40 and OX40 Ligand（OX40L） in children with Henoch-Schonlein purpura（HSP） and explore their possible role and significance of pathogenesis and treatment.Methods Thirty-four HSP children were regarded as research objective and 20 healthy children as healthy control group.Applying reverse transcription-polymerase chain reaction（RT-PCR） technique,using phytohemagglutinin（PHA）,and anti-OX40LmAb as intervention to quantitatively analyze OX40 mRNA and OX40L mRNA expression on peripheral blood mononuclear cells（PBMC）.Results Compared with healthy control group,the expressions of OX40 mRNA and OX40L mRNA both increased obviously in HSP group（Pa〈0.01）.After added in PHA,the expressions of OX40 mRNA and OX40L mRNA in all objective increased obviously（Pa〈0.01）,especially in HSP group（Pa〈0.01）,and then decreased obviously after added in anti-OX40LmAb（Pa〈0.01）.Conclusions The expressions of OX40 mRNA and OX40L mRNA in children with HSP increased abnormally,and the costimulatory molecules OX40/OX40L may play a critical role in the pathogenesis and development of HSP.Blocking the costimulatory pathway appears to be a promising approach of the therapy of HSP.