大蒜油对小鼠酒精性脂肪肝预防作用

Protective effect of garlic oil on alcoholic fatty liver disease in mice

目的探讨大蒜油对小鼠酒精性脂肪肝的预防作用。方法 90只小鼠随机分为6组,每组15只,用乙醇灌胃加高脂饲料复制酒精性脂肪肝模型,大蒜油低、中、高剂量与凯西莱组分别给予相应浓度大蒜油与凯西莱,正常对照组、模型组分别灌胃等体积玉米油,每天1次。连续给药3周后处死动物,检测血生化、病理等有关指标。结果与模型组比较,大蒜油高剂量组血清中总胆固醇(TC)、谷草转氨酶(AST)、谷丙转氨酶(ALT)以及丙二醛(MDA)的水平分别下降了22.42%,25.51%,12.43%,26.27%(P<0.01或P<0.05);肝组织中的TC、甘油三酯(TG)、游离脂肪酸(FFA)、MDA的含量分别降低了25.00%,27.85%,41.13%,60.96%(P<0.01),谷胱甘肽(GSH)含量增加了100.00%(P<0.01);病理检查结果显示,大蒜油各剂量组肝细胞脂滴蓄积明显减少,脂肪变性程度减轻;大蒜油低剂量组的作用程度与凯西莱组相当。结论大蒜油对小鼠酒精性脂肪肝具有预防作用。

Objective To explore the protective effect of garlic oil on alcoholic fatty liver.Methods Ninety mice were divided into six groups（15 in each group）.Alcoholic fatty liver model was established by high fat diet plus ethanol.The mice of garlic oil groups were administered orally with different doses of garlic oil once a day,while the control and model group animals were treated with the same volume of corn oil.At the end of 3 weeks,liver pathologic changes together with the levels of serum alanine aminotrans ferase（ALT）,aspartase amino-transferase（AST）,total cholesterol（TC）,malon-daldehyde（MDA） and the contents of liver MDA,glutathione（GSH）,superoxide dismutase（SOD）,TC,triglyceride（TG）,and free fatty acid（FFA） were examined.Results Compared with the model group,the levels of TC,AST,ALT,and MDA in serum of high doses garlic oil groups were decreased by 22.42%,25.51%,12.43% and 26.27%（P 0.01 or P 0.05）,and the levels of TC,TG,FFA,MDA in liver tissue were decreased by 25.00%,27.85%,41.13%,60.96%,and the level of GSH was increased by 100.00%（P 0.01）.The hepatic steatosis degrees were attenuated obviously.The effect of low dose group was similar to that of tiopronin group.Conclusion Garlic oil has protective effect on alcoholic fatty liver in mice.