家蝇蛆粉对小鼠急性毒性及致突变性作用

Acute oral toxicity and mutagenicity of powder extracted from larvae of Musca domestica in mice

目的探讨家蝇蛆粉对小鼠急性毒性和致突变性,以评价其安全性。方法将家蝇3龄幼虫超声破碎的内容物溶液制成干粉,余下的幼虫壳制成壳聚糖,两者按10∶1比例进行组合混匀,制成粉末状家蝇蛆粉。以昆明小鼠为实验对象,采用最大耐受量实验、骨髓微核实验和精子畸形实验,评价家蝇蛆粉对小鼠的急性毒性和致突变性作用。结果小鼠对家蝇蛆粉的最大耐受量为33.0 g/(kg.bw),未见毒性效应或不良反应;家蝇蛆粉2.8,5.5和11.0剂量组雄性小鼠的嗜多染红细胞微核率分别为0.12%,0.14%,0.14%,雌性小鼠的嗜多染红细胞微核率分别为0.14%,0.12%,0.16%,与溶剂对照组比较,差异无统计学意义(P>0.05),小鼠精子畸形率分别为2.3%,1.6%,2.1%,与溶剂对照组比较差异无统计学意义(P>0.05)。结论家蝇蛆粉对小鼠无急性毒性和致突变性。

Objective To evaluate acute oral toxicity and mutagenicity of powder extracted from larvae of Musca dom-estica（PLMD） for safety assessment.Methods Larvaes of Musca domestica were smashed in the solution with ultrasound.The solution was vacuum-dried into powder and the shells was processed into chitosan.The powder and chitosan were mixed with PLMD at the ratio of 10 vs 1.Acute toxicity test,mouse bone marrow cell micronucleus test and mouse sperm abnor-mality test were conducted.Results The oral maximum tolerated dose of PLMD was more than 33.0 g /kg body weight for mice.The polychromatic erythrocyte micronucleus rate of the male mice treated with PLMD at the doses of 2.8,5.5,and 11.0 g /kg.bw was 0.12%,0.14%,and 0.14,and those of female mice was 2.3%,1.6%,and 2.1%,respectively.The differences were of no statistical significance.Conclusion The PLMD has no acute oral toxicity and mutagenicity effects on mice.