酸枣仁汤对老年失眠证候模型大鼠学习记忆及脑内自由基、一氧化氮合酶的影响

Effects of wild jujube seed decoction on learning memory and levels of free radical and NOS in the brain of the pathogeny model rats with sleep deprivation

目的 探讨酸枣仁汤对失眠证候模型大鼠学习记忆及脑组织丙二醛（MDA）含量及超氧化物歧化酶（SOD）、一氧化氮合酶（NOS）活性的影响.方法 用D-半乳糖致亚急性衰老、环磷酰胺及氧化可的松致阴血亏虚,及自制改良多平台法剥夺睡眠,制作老年阴血亏虚型失眠证候大鼠复合模型,观察大鼠学习记忆、大脑皮质及海马部位MDA含量及SOD、NOS活性的变化,以及酸枣仁汤对这些指标的影响.结果 与环境对照组[（47.3 ±4.6）s,（9±1.4）s,（6.5±1.2）次]比较,证候模型组[（108.9±12.5）s,（89±11.5）s,（0）次]睡眠剥夺48 h后,大鼠学习记忆能力显著下降（t=4.36,3.18,2.07,P＜0.01）,证候模型组皮质、海马部位MDA含量和SOD、NOS活性[（3.8±0.6）、（3.0±0.5）nmol·mgprot-1,（229.7±25.8）、（236.3±25.2）U·mgprot-1,（5.7±0.8）、（5.4 ±0.9）U·mgprot-1]相对于环境对照组[（2.1±0.4）、（1.6±0.4）nmol·mgprot-1,（155.5±10.6）、（147.2±26.1）U·mgprot-1,（2.8±0.7）、（2.9±0.5）U·mgprot-1]均升高（t=2.89,3.01,6.78,5.94,3.10,3.46,P＜0.05）;经酸枣仁汤高、低剂量干预后大鼠学习记忆能力得以明显改善（P＜0.05）,高剂量组皮质、海马部位MDA含量和SOD、NOS活性明显下降（P＜0.05）,低剂量组也明显下降（P＜0.05）,并趋于正常范围.结论 酸枣仁汤对老年失眠证候模型大鼠的学习记忆能力障碍有改善作用,其机理之一可能与减轻睡眠剥夺中自由基和NO对神经细胞的损伤有关.

Objective To observe the effects of wild jujube seed decoction （WJSD） on learning memory and the levels of malondialdehyde （MDA） and the activities of supemxide dismutase （SOD） and nitric oxide synthase （NOS） in the brain of the pathogeny model rats with sleep deprivation （SD）. Methods Levels of learning memory and MDA, and activities of SOD and NOS in rats＇ brain were assayed after SD induced by made-self multiple platform method （ MMPM）, senescence induced by D-galactose, and Yin and blood-deficiency induced by eyclophosphamide and hydrocortisonum. Results After 48 h SD, the levels of learning memory was lower in the model group（ （ 108.9 ± 12.5 ） s, （ 89 ± 11.5 ） s, （ 0 ） ） than those in the environmental control group （ （ 47.3 ±4.6）s,（9±1.4）s,（6.5 ±1.2））（（ t=4.36,3.18,2.07, P〈0.01==. While MDA, and activities of SOD and NO in rats＇brain higher in the model group（ （3.8 ±0.6） ,（3.0 ±0.5）nmol · mgprot-1 ,（229.7 ±25.8） ,（236.3± 25.2 ） U · mgprot - 1, （ 5.7 ± 0. 8 ）, （ 5.4 ± 0.9 ） U · mgprot - 1 ） than those in the environmental control group （ （2. 1 ±0.4） ,（1.6 ±0.4）nmol · mgprot-1 ,（155.5 ±10.6） ,（ 147.2 ±26.1 ）U · mgprot-1 ,（2.8 ±0.7）,（2.9 ± 0.5 ） U · mgprot -1 ） （ t = 2.89,3.01,6.78,5.94,3.10,3.46, P 〈 0.05 =. However, the observation of the groups treated with WJSD, the levels of learning memory showed a tendency in returning to normal level （P 〈 0.05 = ,and MDA, and activities of SOD and NO of the high dose of WJSD showed a tendency in returning to normal leve1 （P 〈 0.05 = ,and the low dose of WJSD showed a tendency in returning to normal level too（P 〈 0.05 =. Conclusion WJSD can improve the disability of learning and memory of the pathogeny rats model, and its one of mechanism maybe involve the reduction of neural cell damage with free radical and NO.