不同居属地半夏核苷类有效成分的比较研究

Comparative study on active ingredients of tubers of Pinellia ternata (Thunb.)Breit.from different populations

目的:对全国不同居群半夏进行初步的质量评价,为充分利用和开发半夏资源提供科学依据。方法:收集不同居群的半夏品种,以其有效成分(核苷类成分)含量为评价指标,进行品种质量评价,以遴选出优良半夏品种。总核苷类成分含量(以鸟苷计)采用高效液相色谱法(HPLC)测定。结果:不同居群半夏药材的总核苷类成分存在显著性差异,江苏泰半夏的核苷类成分含量显著高于其他居群。结论:我国半夏种质参差不齐,要培育出优质半夏,需要通过各种人工方法对品质优良的半夏进行品种选育。

Objective： To evaluate the quality of Rhizoma pinelliae from different populations and provide the scientific basis for using and exploiting resources of Rhizoma pinelliae fully.Methods： To collect Rhizoma pinelliae from different populations,chose their active ingredient（nucleotide composition） content as the indicators for the quality evaluation,selected the excellent breed from them.Furthermore,adopt the method of High-Performance Liquid Chromatography（HPLC） for the content of the nucleotide composition（with guanosine） measurement.Results： The content of nucleotide composition from different populations were significantly different.Rhizoma pinelliae from Taizhou city was much higher than other populations on the content of nucleotide composition.Conclusion： In our country,the quality of Rhizoma pinelliae from different populations is varied.In order to produce high-quality Rhizoma pinelliae,it′s necessary to create the breed of Rhizoma pinelliae which gathers various advantages through some artificial methods.