大孔树脂对柚子内果皮柚皮苷的分离纯化

Isolation and Purification of Naringin from Citrus grandis Peel by Macroporous Resins

对大孔树脂对柚子内果皮柚皮苷的分离纯化进行了研究,通过静态吸附-解吸实验,从5种大孔吸附树脂中筛选出具有较大吸附量和解析率的D3520树脂为层析柱的填充料,树脂D3520静态吸附量达到12.15mg/g树脂,乙醇洗脱率为73.94%。对柚皮苷的粗提液进行纯化工艺的研究,得到柚皮苷的最佳纯化条件:吸附原液的浓度约为28μg/mL,pH值7;洗脱剂选择pH=5的70%乙醇,洗脱剂用量为100mL,洗脱流速为1mL/min。在此条件下得到的洗脱液采用低温冷冻干燥的方法,得到淡黄色粉末。利用紫外光谱定性分离纯化物具有柚皮苷的吸收光谱特征。利用HPLC定性分离纯化物为柚皮苷,含量为85.66%。

Through static absorption performance and disabsorption performance experiments,macroporous resin D3520 was chose as the absorption carrier for isolation and purification of naringin from Citrus crandis peel.The static absorption quantity of this resin can reach 12.15 mg/g dry resin and elution rate was 73.94%.The crude extracts was purified by using the D3520 absorption resin under the following conditions：flow rate 1mL/min,under-adsorpted solution concentration 28 μg/mL,the pH value of under-adsorpted soulution 7,the elution solvent 50% alcohol,pH value of elution solvent 5,and the quantity of elution solvent 100 mL.Then the eluent was dehydrogenized by vacuum-freeze drying method,giving the tan powder of naringin.The absorption spectrum of flavonoids in product detected by ultraviolet spectrometer had the characteristics of naringin.And HPLC analysis showed that the flavonoids in the product belong to naringin.