潞党参多糖的提取及含量测定

Extraction and determination of the polysaccharide in radix codonpis pilosulae

目的:从规范化产区潞党参中提取多糖并测定其多糖的含量。方法:通过水提醇沉法,提取潞党参中的多糖,并用苯酚-硫酸法测定其多糖含量。结果:测得潞党参中多糖含量为20.99%,平均回收率为98.43%,RSD=1.03%(n=6)。结论:潞党参中具有较高多糖量,是一种具有开发利用价值的中药材。

Objective：To extract the polysaccharide and measure the polysaccharide content in Radix Codonopsis pilosu lae from good agriculture practice（GAP） areas.Methods：Polysaccharide was extracted from Codonopsis with ultrasonic technique,and its content in Codonopsis was determined with phenolsulfuic acid method.Results：The polysaccharide content in Radix Codonopsis pilosulae was 20.99%,the rate of recovery was 98.43%,and RSD was 1.03%（n=6）.Conclusion：Codonopsis has higher content of polysaccharide,and it has value of utilization and development.