气虚发热证家兔皮肤、肺脏、脾脏的形态学变化

The morphologic changes of skin,lung and spleen in rabbits with syndrome of fever due to qi deficiency

目的研究气虚发热证家兔的皮肤、肺脏、脾脏的形态学变化,探讨气虚发热对皮肤、肺脏、脾脏的影响。方法将家兔随机分成2组,即正常组与气虚发热组,采用禁食不禁水和腹腔注射脂多糖(LPS)建立气虚发热模型,取皮肤、脾脏、肺脏组织制备石蜡组织切片,显微镜下观察其形态学变化。结果显微镜下观察组织切片,与正常组相比,气虚发热组家兔肺脏局部有炎性细胞浸润,脾脏局部组织有灶状多核巨细胞浸润并见肉芽肿性病变,皮肤表皮呈不规则皱缩,毛囊变短,毛孔头数明显减少。结论气虚发热证家兔的皮肤、脾脏、肺脏与正常家兔比较存在组织形态学差异。

Objective To investigate the morphologic changes in skin,lung and spleen of rabbits with syndrome of fever due to qi deficiency,and to explore the effect of syndrome of fever due to qi deficiency on skin,lung and spleen.Methods Rabbits were randomly divided into two groups,normal group and group of syndrome of fever due to qi deficiency.The syndrome of fever due to qi deficiency group was developed by administering LPS and fasting.Paraffin sections of skin,lung,and spleen were prepared after hematoxylin and eosin（HE） staining,and morphological changes were observed under light microscopy.Results Compared with the normal tissue sections from the control group,local inflammatory cell infiltration from lung tissue,infiltration of focal multinucleated giant cells and granulomatous lesion from spleen,and irregular wrinkling of epidermis,hair follicle shortening were observed in the groups of rabbits with syndrome of fever due to qi deficiency,with significant decreasing number of skin pore visible under light microscopy.Conclusion There were morphological differences in microscopic structures of skin,lung,and spleen between the healthy rabbits and those with syndrome of fever due to qi deficiency.