行瘀清热胶囊对大鼠前列腺成纤维细胞增殖及凋亡的影响

The effects of Xinyuqingre capsules on proliferation and apoptosis of prostatic fibroblasts of rats

目的观察行瘀清热胶囊对大鼠前列腺成纤维细胞增殖及凋亡的影响。方法取健康SD大鼠前列腺组织进行成纤维细胞的原代培养并传代增殖,选用第3～6代细胞,制备行瘀清热胶囊含药血清,于酶标仪490 nm波长处测定吸光度(A)值,计算药物对成纤维细胞的相对抑制率,并以流式细胞仪分析行瘀清热胶囊对前列腺成纤维细胞的凋亡率的影响。结果行瘀清热胶囊能抑制前列腺成纤维细胞增殖;流式细胞仪分析显示行瘀清热胶囊含药血清使成纤维细胞凋亡率增高较为明显。结论行瘀清热法防治慢性前列腺炎的机制与抑制前列腺组织成纤维细胞增殖、促进成纤维细胞的凋亡有关。

Objective To investigate the effect of Xinyuqingre capsules on proliferation and apoptosis of prostatic fibroblasts of rats.Methods Serum containing active ingredients of Xinyuqingre capsules was applied to the 3 rd～6 th generation of prostatic fibroblasts of healthy rats.The optical absorbance was determined with ELISA at the wavelength of 490 nm and the relative inhibition ratio was calculated.The effects of Xinyuqingre capsules on the apoptosis index of prostatic fibroblasts were determined by flow cytometer.Results Xinyuqingre capsules could inhibit the proliferation of prostatic fibroblasts of rats,flow cytometric analysis demonstrated that the serum containing active ingredients of Xinyuqingre capsules could increase the apoptosis rate of prostatic fibroblasts of rats.Conclusion The mechanism of Xinyuqingre method to treat chronic prostatitis was related to the inhibition of the proliferation and increasing of apoptosis rate of prostatic fibroblasts.