应用RT-PCR方法快速诊断猪繁殖与呼吸综合征的研究

Rapid Diagnosis of Porcine Reproductive and Respiratory Syndrome with the RT-PCR

参照国内外已发表的猪繁殖与呼吸综合征病毒(PRRSV)的ORF5基因序列设计1对引物,建立检测PRRSV的RT-PCR方法,并对长春周边不同地区10个猪场送检的20份病料进行检测。特异性试验、敏感性试验和重复性试验结果表明,此方法可以用于临床检测PRRSV。应用该方法从3个猪场的4份病料检出PRRSV,证实长春周边地区存在PRRSV感染。

This research has designed a pair of primers based on the ORF5 gene of the PRRSV sequences published to set up a RT - PCR method in order to detect PRRSV. We have detected 20 pathologic materials via this method from 10 different pig farms around Changchun. It has been indicated that the detecting method of RT- PCR can apply to clinical detection through specificity test, sensitivity test and repeatability test. The results of this research detected 4 pathologic materials PRRSV positive from 3 farms confirming that PRRSV infection does exist around Changchun.