痰液脱落细胞中RASSF1A基因甲基化与肺癌关系的研究

The Study of the Association between Lung Carcinoma and the Methylation of RASSF1A Gene in Desquamated Cells from Sputum

目的主要对肺癌患者谈脱落细胞中RASSF1A基因甲基化的状态进行分析和研究,对其在帮助诊断肺癌过程中的作用进行探讨。方法采用甲基化特异性PCR检测肺癌、肺癌高危人群、正常人群痰脱落细胞中RASSF1A基因甲基化发生状态,分析与临床指标之间的关系及差异。结果肺癌中该基因16.7%出现甲基化;肺癌高危人群中只有6%出现甲基化;正常人群中该基因无甲基化。RASSF1A基因甲基化和患者年龄、性别、吸烟程度、组织分型和临床分期均无相关性。讨论RASSF1A基因甲基化的发生率,在肺癌患者身上发生的概率与普通人身上发生的概率相比,要高出很多,同时,经过研究发现,RASSF1A基因甲基化的发生率与患者的性别和年龄等因素无关,与肿瘤组织的分型也没有相关性,因此,在临床上,RASSF1A基因甲基化在确诊肺癌的过程中,具有很大的帮助作用。

Objective Mainly for patients with lung cancer cells exfoliated RASSF1A gene talk of methylation condition are analyzed and studied, in its help diagnose lung cancer process function are discussed. Methods Adopt methyl lation specifi city PCR detection lung cancer, lung high-risk groups, normal crowd phlegm RASSF1A gene in cells exfoliated methyllation happen state, analysis and the relationship between clinical index and differences. Results Lung cancer in the gene 16.7% appear methylation, Lung cancer in high-risk only 6% appear methylation, Normal crowd the gene without methylation. RASSF1A gene methylation and patient age, sex, smoking status, organization classification and the clinical stages were unrelated. Conclusion RASSF1A gene methylation the incidence of lung cancer patients in the odds of more than ordinary chance of happening, compared to a much higher, at the same time, after the study found that the incidence of RASSF1A gene methylation with patients gender and age factors have nothing to do with tumor tissue the parting no correlation, therefore, in clinical, RASSF1A gene methylation is diagnosed lung cancer, in the process of great help.