摘要
探讨血管生成抑制多肽HM-3和ES-2在组织中分布的特点。将两种多肽HM-3和ES-2分别用异硫氰荧光素（FITC）进行标记,建立C57BL/6黑色素瘤（B16F10）小鼠模型,将标记多肽以尾静脉注射给药,通过流式细胞仪检测分析给药后小鼠各个组织中多肽的分布情况。结果表明血管生成抑制多肽HM-3和ES-2主要分布在肝、肾和胃中,且分布浓度会随着时间的推移而降低,在心、脾和肺中基本没有分布。
The article mainly discussed the distribution of the anti-angiogenic polypeptides HM-3 and ES-2 in different tissues.Two peptides were marked with fluorescein isothiocyanate（FITC）.The distribution of peptide in various organs was analysised by flow cytometry（FCM）after being injected into the tail vein of C57BL/6 melanoma（B16F10）model mice.The results showed that the distribution of the anti-angiogenic polypeptides HM-3 and ES-2 mainly in liver,kidney and stomach.And it decreased with the time extended.However,few HM-3 and ES-2 can be detected in heart,spleen or lung.
出处
《药物生物技术》
CAS
CSCD
2010年第5期417-420,共4页
Pharmaceutical Biotechnology
基金
国家重大新药创制科技重大专项（项目编号：200909102-244）
