水稻糖基水解酶基因GH27启动子克隆及初步分析

Cloning and Preliminary Analysis of Promoter of Rice Glycosyl Hydrolase Gene GH27

通过生物信息学方法找到水稻中与拟南芥β-葡糖苷酶类基因同源的糖基水解酶基因GH27。Northern检测结果显示该基因在衰老晚期的叶片中高量表达。利用PCR技术克隆GH27基因的启动子(PGH27)序列,构建启动子PGH27与报告基因GUS融合基因的表达载体PGH27:GUS,并通过农杆菌介导法转化水稻品种中花11,GUS染色结果表明该启动子PGH27可以有效地驱动GUS基因在转基因愈伤组织和水稻植株老叶中表达。

A glycosyl hydrolase gene GH27 which was associated with rice senescence was identified by bioinformation technology.The expression level of GH27 increased in late senescent leaves through Northern blot analysis which indicated the promoter PGH27 may be senescence specific.PGH27 was cloned using PCR method,and the sequence was analyzed.An expression vector containing the PGH27：GUS fusion gene was constructed and transferred into rice variety＂Zhonghua 11＂by Agrobacterium-mediated method.A strong expression of GUS was observed in both transgenic calli and senescing leaves.