HPLC法测定布渣叶中山柰素的含量

Determination of Kaempferol in Microcos Paniculata by HPLC

目的:建立布渣叶中山柰素的含量测定方法,为其制定质量标准提供依据。方法:采用高效液相色谱法对布渣叶药材山柰素进行含量测定。色谱柱:Diamonsil(钻石)C18(250×4.6mm,5μm);流动相:甲醇-0.4%磷酸溶液(52∶48);流速:1.0mL/min;柱温:30℃;检测波长:367nm。结果:山柰素进样量在0.01021ug^0.20420ug范围内与峰面积呈良好的线性关系(r=0.9999)。平均加样回收率为99.13%,RSD为1.05%(n=6)。结论:所建立的方法简便、快捷,重现性好,可用于该药材的质量评价。

Objective：To establish the quantitative methods of kaempferol in Microcos paniculata and control its quanlity.Methods：The content of kaempferol in Microcos paniculata was determined by HPLC.The chromatgraphic conditions were as follows：Diamonsil C18（250×4.6mm,5μ）,mobile phrase was methanol-0.4% phosphoric acid（52∶48）,flow rate being 1.0ml/min,column temperature at 30℃,detection wavelength being 367nm.Results： Kaempferol showed a good linear relationship in the range of 0.01021ug^0.2042ug（r=0.9999）,the average recovery was 99.13% and RSD was 1.05%.Conclusion：The established methods are simple and rapid with good reproducibility,and can be used for the quality control of Microcos paniculata.