摘要
克隆了与草莓成熟有关的乙烯受体Etr1基因片段,为进一步研究Etr1基因功能并通过基因技术改善草莓贮运性能奠定基础。以全明星草莓成熟果实中分离到得基因组DNA为模板,经PCR扩增到一条约600bp的特异片段,将该片段克隆到pEGM-T easy vector上经测序分析,基因全长617bp,编码205个氨基酸。序列分析结果表明,该序列与Chandler-Etr1的cDNA序列同源性98%,氨基酸序列同源性97%。
In this study a ripening related ethylene recepor Etr1 gene in strawberry fruits was cloned for the following-up reseach on gene and improving varieties of strawberry through transgenic technology. A pair of primers were designed and synthesized based on the sequence reported of Chandler-Etr1. Using genome DNA extracted from All-star strawberry mature fruits as template the specific PCR product of Etr1 was obtained,then it was ligated to pEGM-T eas y vector and sequenced. The sequencing data showed that the PCR product was 617 bp encoding 205 predicted amino acid residues. Comparison with the cDNA sequence from Chandler-Etr1 indicated the homlogy was 98% ,and amino acid identitise were 97% .
出处
《华北农学报》
CSCD
北大核心
2010年第5期68-71,共4页
Acta Agriculturae Boreali-Sinica
基金
河北省科技攻关项目(04395501D-3)
