摘要
为了分离克隆强旱生植物沙冬青的ERF基因,研究其结构和功能,在已对沙冬青cDNA文库和EST分析的基础上,设计了1对特异性引物,利用PCR技术分离克隆了1个沙冬青ERF基因。该基因全长899bp,没有内含子,开放阅读框633bp,编码211个氨基酸,编码的蛋白理论MW=23216.0,理论pI=8.20。
To study the structure and function of ERF,one pairs specific primers were designed,then a new gene AmERF was isolate and cloned from Ammopiptanthus mongolicus genome by PCR technology based on analysis of cDNA Libraries and EST of AmERF. The results indicated that the full-length of AmERF DNA was 899 bp,without intron,had an open reading frame of 633 bp,encoding a predicated polypeptide of 211 amino acids with a molecular weight of 23. 2 kDa and a calculated pI of 8. 2. The study of AmERF gene in the process of biotic stress could provide effective candidate genes for resistance breeding of crop.
出处
《华北农学报》
CSCD
北大核心
2010年第5期76-79,共4页
Acta Agriculturae Boreali-Sinica
基金
国家甜菜现代产业技术体系项目
关键词
沙冬青
ERF基因
克隆
Ammopiptanthus mongolicus
ERF gene
Cloning
