基于叶绿体DNAtrnL-F序列研究部分鸢尾属的亲缘关系

Relationship of Iris Inferred from the Chloroplast DNA trnL-F Sequence Variation

利用通用引物,采用PCR技术从24个鸢尾样品中扩增了叶绿体基因组trnL-F间隔区的DNA片段,进行了序列测定,序列长度为1003～1113bp,比较长度为954bp,其中共有92个位点发生了单个碱基的置换,14个位点发生了缺失,因此有进化意义的位点共106个,约占总序列比较长度的11.11%。运用DNAMAN软件分析了这些鸢尾的亲缘关系,可分为2个相对独立的大组。第一大组可以分为2组:3个种源马蔺关系很近形成一组,粗根鸢尾、溪荪和喜盐鸢尾聚成一组。第二大组也分为2组:6个种源的马蔺与野鸢尾聚在一起,再和小鸢尾聚成一组;3个种源的鸢尾、高原鸢尾、德国鸢尾、矮鸢尾、野鸢尾、扇形鸢尾、红籽鸢尾和I.ungiucularis聚成一组。

The intergenic region of trnL and trnF was amplified by PCR for 24 samples of Iris. The lengths of the segments varied from 1 003 bp to 1 113 bp,of which 954 bp was compared. The number of the evolutionary loci was 106,including 92 transpositional loci and 14 missing ones,which was 11. 11% of the compared lengths. Their relation-ships were performed using software DNAMAN. It showed that all the samples were divided into two parts. The first part included two groups,one was I. lactea from Taipu,Xiwuqi and Wulumuqi,the other included I. tigridia,I. sanguinea and I. halophila. The second part included two groups. One group included I. lactea from Beijing,Minqin, Guyuan,Taishan,Wuwei and Zhuozhou,I. dichotoma and I. proantha. The other group included I. tectorum,I. collettii, I. germanica,I. pumila,I. dichotoma,I. wattii,I. foetidissima and I. ungiucularis.