尖锐湿疣组织中Survivin蛋白表达与角质形成细胞凋亡的相关性及意义

Relationship bewteen the expression of of Survivin protein and the apoptosis of keratinoeytes and its significance in condyloma acuminatum

目的探讨Survivin蛋白在尖锐湿疣(CA)发生、发展中的作用。方法采用免疫组化和TUNEL技术检测56份CA组织(观察组)中Survivin蛋白表达和角质形成细胞凋亡指数,并与25份正常包皮组织(对照组)比较。结果观察组及对照组Survivin蛋白阳性率分别为64.29%(36/56)、4.00%(1/25),P<0.01;角质形成细胞凋亡指数分别为(9.73±3.51)%、(0.95±0.16)%,P<0.01;观察组Survivin蛋白表达阳性者及阴性者角质形成细胞凋亡指数分别为(6.16±2.58)%、(14.31±14)%,P<0.05。Survivin蛋白表达与凋亡指数呈负相关(r=-0.694,P<0.05)。结论 CA组织中存在Survivin蛋白过表达,可抑制角质形成细胞凋亡,促进CA的发生及发展。

Objective To explore the role of Survivin protein in the occurrence and development of condyloma acuminatum（ CA） . Methods The expression of Survivin protein and apoptosis index of keratinocytes in 56 cases of condyloma acminatum tisuses（ observed group） were detected using the technology of immunohistochemistry and TUNEL,then compared with those in 25 cases of normal skin tissues（ control group） . Results The positive rate of Survivin protein were 64. 29% （ 36 /56） ,4. 00% （ 1 /25） in observed group and control group respectively,P 0. 01; the apoptosis index of keratinoeytes were （ 9. 73 ± 3. 51） % ,（ 0. 95 ± 0. 16） % respectively,P 0. 01; the apoptosis index of the cases with positive and negative expression of Survivin protein in observed group were （ 6. 16 ± 2. 58） % ,（ 14. 31 ± 5. 14） % respectively,P 0. 05. The expression of Survivin protein was negatively correlated with the apoptosis index （ r =-0. 694,P 0. 05） . Conclusion There is over expression of Survivin protein in CA tissues,which can inhibit the apoptosis of keratinocytes and improve the occurrence and development of CA.