美国红枫组织培养与快繁技术的研究

Tissue Culture and Rapid Propagation of Acer rubrum

以美国红枫嫩茎为外植体,研究不同培养基对美国红枫组织培养的影响。结果表明,不同浓度的激素组合对红枫组织培养有不同的影响,最佳诱导培养基为1/2MS+IAA0.3mg/L+6-BA0.6mg/L;最佳增殖培养基为1/2MS+NAA0.2mg/L+6-BA2.0mg/L;生根培养最适培养基为MS+NAA0.5mg/L。NAA、IAA及6-BA等激素组合对红枫不定芽诱导、增殖和丛生芽生根培养有较大的影响。

The tendergreen stems were used as explants to study the effect of different medium on tissue culture of Acer rubrum.The results showed that there were different impacts on Acer rubrum＇s tissue culture in different concentrations of hormone combinations.The optimal induction medium was 1/2MS＋IAA 0.3 mg/L＋6-BA 0.6 mg/L.The optimal proliferation medium was 1/2MS＋NAA 0.2 mg/L＋6-BA 2.0 mg/L.The adaptive rooting medium was MS＋NAA 0.5 mg/L.Concentrations of hormone combinations such as NAA,IAA and 6-BA had great effects on adventitious buds induction,proliferation and rooting of Acer rubrum.