摘要
本文提出了基于数字图像处理和精密机械动作大于光学显微镜观察视野(0.647mm×0.483mm)的微小物体的三维重构方法。该方法首先运用光学聚焦原理采集不同焦平面上的图像组序列,采用图像拼接算法,拼接出研究对象的整体图像。然后采用图像块分割算法和灰度差分算子对图像序列进行分析。最后结合聚焦合成的叠合算法快速地实现了微小物体的三维重构。实验表明,该方法简单有效,所生成的三维重构效果图提供了微小物体的更加丰富的信息。
This paper reports a novel 3D（three dimensional） reconstruction method from optical microscopic images.The method can effectively reconstruct 3D microstructures based on digital image processing and precision mechanics,greater than the field of view of an optical microscope of 0.647 mm × 0.483 mm.Firstly,partial-sample images are taken on different focal planes for the whole microstructure.Those partial-sample images are merged to create a sequence of whole-sample images of different focal planes.The sequence of whole-sample images is spliced to keep only the area of interests by using a highly efficient image splicing methods.The spliced images sequence is then used in 3D reconstruction of the microstructure by a coincidence algorithm.Experimental results show that the method is simple and effective,and the 3D reconstructed image provides richer information of the microstructure.
出处
《光电工程》
CAS
CSCD
北大核心
2010年第11期84-90,共7页
Opto-Electronic Engineering
关键词
三维重构
焦平面
图像拼接算法
叠合算法
3D reconstruction
focal planes
image splicing methods
coincidence algorithm
