运输应激对食蟹猴血清生化指标、丙二醛及总抗氧化能力的影响

Influence of Transportation Stress on Serum Biochemical Parameters,Malondiadehyde and Total Antioxidant Capacity in Cynomolgus Monkeys

目的研究食蟹猴在长途运输后血浆皮质醇、血清生化指标、丙二醛(MDA)及总抗氧化能力(TAOC)变化情况。方法对雄性食蟹猴在运输前后不同时间采血,进行血清生化指标、血清MDA及TAOC测定。结果运输后ALT、AST及LDH显著升高(P<0.05),BUN、CREA、TP及ALB显著下降(P<0.05);ALP、GLU及TBIL升高,TCHO及CK下降,但差异均不显著。除CK与LDH外,其余指标均在第29天时恢复正常。运输后血清MDA逐渐升高(P<0.01),第2天达到峰值,第29天趋于稳定;TAOC逐渐降低,第2天降至最低(P<0.05),第29天趋于稳定。结论运输不仅可引起多个血清生化指标发生变化,还可以使食蟹猴处于氧化应激状态中;运输后至少应该有29天的适应饲养期,以保证血清生化指标恢复到正常水平及机体氧化与抗氧化状态恢复平衡。

Objective To evaluate the influence of long distance transportation on plasma cortisol, serum biochemical pa- rameters, malondiadehyde （MDA） and total antioxidant capacity （ TAOC ） in cynomolgus monkeys （ Macaca fascicularis）. Methods Blood samples were collected from male cynomolgus monkeys at different time points of pre- and post-transportation and were used to determine serum biochemical parameters, MDA and TAOC. Results ALT, AST and LDH increased significantly after transportation （P 〈 0.05）. ALP, GLU and TBIL increased but there was no significant difference. BUN, CREA, TP and ALB decreased significantly after transportation （ P 〈 0.05 ）. TCHO and CK decreased but there was no sig- nificant difference. Except for CK and LDH, all serum biochemical parameters returned to normal levels on the twenty-ninth day. Serum MDA increased gradually after transportation, reached a peak value on the second day （ P 〈 0.01 ） and became stable on the twenty-ninth day. Serum TAOC decreased gradually after transportation, reaehed the trough value on the sec- ond day （P 〈 0.05） and became stable on the twenty-ninth day. Conclusions Transportation stress could affect the values of most of the serum biochemical parameters and cause oxidative changes in cynomolgus monkeys after transportation. At least, an adaptation period of 29 days should be guarantied for the serum biochemical parameters to return to their normal values and the oxidative versus anti-oxidative status get back to their balance.