过敏性紫癜患儿急性期T细胞亚群与自然杀伤细胞活性的研究

Study of T-cell dysfunction and NK cells activity in acute phase of Henoch-Schonlein purpura in children

目的研究过敏性紫癜(HSP)患儿急性期外周血淋巴细胞亚群的变化及其临床意义。方法采用流式细胞仪(FCM)检测53例HSP患儿和20例健康查体儿童外周血淋巴细胞亚群CD3+、CD4+、CD8+、CD4+/CD8+、CD19+和NK(CD16++CD56+)。结果 HSP患儿与对照组儿童比较,HSP患儿急性期外周血CD4+、CD4+/CD8+和NK(CD16++CD56+)细胞水平明显下降(P<0.01);CD8+、CD19+百分率明显升高(P<0.05)。结论过敏性紫癜患儿急性期存在T细胞功能紊乱和NK细胞活性下降。

Objective To investigate immunological function in acute phase of Henoch-Schonlein purpura（HSP） in children,in order to explore the role of T cells subsets of HSP.Methods Choose Fifty-three children with HSP in acute phase were enrolled in this study,twenty healthy children were control group.Detect the CD3^＋,CD4＋,CD8^＋,CD4＋/CD8^＋,CD19^＋,and the NK cells activity by flow cytometry（FCM）.Results The percentage of CD4＋、CD4＋/CD8^＋and NK（CD16^＋＋CD56^＋）cell in HSP group were significantly lower than those in control group（P0.01）.The percentage of CD8^＋cell and CD19^＋ cell was significantly higher than that in control group（P0.05）.Conclusions The HSP children appear the T-cell dysfunction,and NK cells activity decreasing.