苹果柱型性状的SRAP分析

SRAP analysis on columnar trait in apple(Malus domestica Borkh.)

为了更深入研究苹果柱型基因,为苹果树型性状改良奠定基础,以舞姿与富士杂交的F1代群体为材料,利用SRAP分子标记技术与BSA法相结合的方法分析了苹果柱型性状。筛选出了1对SRAP引物组合M10E4,其在柱型池与普通型池间表现出分离,分离片段约为310bp。选用柱型与普通型的极端类型进行验证,柱型群体中多数单株都出现此片段,有2株出现交换,普通型群体中有3株出现交换,交换率为7.14%,用Mapmaker/Exp3.0软件对标记与目标性状进行连锁分析,测算遗传距离为7.7cM,并将此标记命名为M10E4-310。研究结果表明,利用SRAP技术初步找到了与柱型基因连锁较紧密的标记位点。

In order to deeply research columnar gene in Malus domestica Borkh.,and lay a foundation for trait improvement of tree type in M.domestica Borkh.,taking the progeny of Fuji×Telamon as materials,columnar trait was analyzed by using BSA method and SRAP molecular marker technology.A SRAP primer combination M10E4was selected out.The primer combination showed separation between columnar and common gene pools,and separation fragment was about 310bp.Most materials had the fragment in columnar phenotype plants,and gene recombination occurred in 2materials;gene recombination occurred in 3materials from general phenotype plants,and the recombination frequency was 7.14%.The analysis result of Mapmaker/Exp 3.0software showed that genetic distance between the marker and COlocus was 7.7cM.The SRAP marker M10E4-310was named.The results showed that the marker locus having more close linkage with columnar gene could be found by using SRAP technique.