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加拿大进境油菜籽加工过程中油菜茎基溃疡病菌的检测与鉴定 被引量:5

Detection and characterization of Leptosphaeria maculans in processed rapeseed imported from Canada
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摘要 从加拿大进境油菜籽中随机选取船载油菜籽、筒仓油菜籽、菜籽粕和下脚料4类加工过程的样品调查油菜茎基溃疡病菌的发生情况。结果表明,18份船载油菜籽、7份筒仓油菜籽和11份下脚料样品的油菜茎基溃疡病菌PCR检测都呈阳性,而8份菜籽粕样品全为阴性。3份阳性样品的病原菌分离、鉴定与致病性测试结果证实了PCR的检测结果。 In this research,four types of samples,including shipborne rapeseed,storage silo rapeseed、rapeseed dregs and byproduct,were randomly selected from processed rapeseeds to investigate the ocurrence of Leptosphaeria maculans.The results showed that all eighteen shipborne rapeseeds,seven storage silo rapeseeds,and eleven byproducts were positive in PCR detection of Leptosphaeria maculans,but eight rapeseed dregs were negative in such detection.The results from pathogen isolation,charcterization and pathogenicity testing of three samples comfirmed the PCR detection.
出处 《植物检疫》 北大核心 2010年第6期25-26,共2页 Plant Quarantine
基金 湖北检验检疫局科研项目(HUBE2010-23)
关键词 油菜籽加工 油菜茎基溃疡病菌 PCR 病原菌鉴定 rapeseed processing Leptosphaeria maculans PCR pathogenic characterization
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参考文献1

  • 1Taylor L Janet.A simple sensitive and rapid method for detecting seed contaminated with highly virulent Leptosphaeria maculans[].Ap-plied and environmental microbiology.1993

同被引文献60

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