摘要
目的为了制备能检测日本血吸虫种内多态性的ｒＤＮＡ探针，以用于不同地域日本血吸虫品系间差异的研究。方法根据曼氏血吸虫大、小亚基的基因序列，设计引物，ＰＣＲ扩增得到４．４ｋｂ的日本血吸虫中国大陆株安徽品系的ｒＤＮＡ片段，经纯化后克隆入ｐＵＣ１８质粒。结果得到了２个转化成功的含日本血吸虫中国大陆株ｒＤＮＡ基因的重组质粒的ＪＭ１０３菌落。结论我们采用的方法，使获取目的基因片段的手段更为简单、方便。
Aim To obtain Schistosoma japonicum(Sj) rDNA probe for research on genetic diversity of the parasite Methods\ We designed a pair of primers according to Schistosoma mansoni rDNA sequence and get the expected Sj specific rDNA which is about 4 4 KB long by PCR,then the amplified fragment was successfully coloned into pUC 18 plasmid vector Results\ Two positive JM103 clones with the recombinant plasmid were perserved for further study Conclusion\ This make it simple and convenience to get the high quality target DNA fragments
出处
《中国人兽共患病杂志》
CSCD
北大核心
1999年第2期24-26,共3页
Chinese Journal of Zoonoses
基金
国家自然科学基金
