PCR快速检测致病性钩端螺旋体DNA

RAPID DETECTION OF LEPTOSPIRA DNA BY POLYMERASE CHAIN REACTION

以四株不同型别的致病性钩端螺旋体ＤＮＡ为模板进行聚合酶链反应（ＰＣＲ），建立了钩体快速检测方法。在此基础上，先后对流行期１５例早期疑似血清进行ＰＣＲ快速检测，结果７例阳性，与经防疫部门及临床确诊的７例患者完全符合，阳性率为１００％。而常规方法（显微镜凝集试验）只检出５例阳性。结果表明，ＰＣＲ用于钩体病的早期论断，快速、准确。

S A method of rapid detecting Leptospira DNA was constructed after the template DNA from 4 serotypes of Leptospira interrogans were successfully amplified by polymerase chain reaction(PCR).Then the method was used to analyse 15 sera samples from uncertain patients 7 samples were detected to be positive,the positive rate was 100%,this method is better than current method of MAT(only 5 samples were positive) The result demonstrated that PCR is an advanced diagnostic technique for early leptospirasis,and it's sensitive,specific and practical