恶性疟原虫乳酸脱氢酶的快速电泳法检测及影响因素

Fast Detection of Lactate Dehydrogenase of Plasmodium Falciparum and Factors Affecting the Results

目的建立一种敏感、特异的恶性疟快速诊断方法。方法：应用快速电泳法检测恶性疟病人血样中恶性疟原虫乳酸脱氢酶（ＬＤＨ－Ｐ），并对可能影响检测结果的几种因素进行观察。结果：检测４０份恶性疟病人血样，３８份检测出ＬＤＨ－Ｐ，阳性率为９５％，假阴性率为５％；同时检测４０份间日疟病人血样和２０份正常人血样，均为阴性，无假阳性；血样反复冻融可使ＬＤＨ－Ｐ受到破坏，蛋白酶抑制剂对保存血样中的ＬＤＨ－Ｐ保护作用明显；新鲜血样中加否蛋白酶抑制剂，检测结果差异不明显。结论：快速电泳法检测ＬＤＨ－Ｐ诊断恶性疟，方法简便、敏感性和特异性高，具有很好的现场应用价值。检测新鲜血样中ＬＤＨ－Ｐ效果好。

Aim To establish a method that can diagnose falciparum malaria quickly Method:The fast electrophoresis method was used to detect lactate dehydrogenase of plasmodium falciparum(LDH-P) from blood samples of patients with plasmodium falciparum with the factors possiblly affecting the results observed Results:The positive coincidence rate in plasmodium falciparum samples was 95% with a false negative coincidence rate of 5% But no LDH-P activity was deteced from either plasmodium vivax or control samples It was not significant that the protection of proteinasa inhibitors to LDH-P from fresh blood samples Repeated freeze and thaw and blood samples stored for a long time were destructive to LDH-P lowing the positive coincidence rate Proteinase inhibitors were found to be protective to LDH-P from stored blood samples Conclusion:It could be a ideal diagnostic method of falciparum malaria to detect LDH-P with fast electrophoresis method from fresh blood samples