氟中毒大鼠肝细胞凋亡研究

Hepatocyte apoptosis in fluorosis rats

目的研究氟化物对肝细胞凋亡的影响。方法用偏食饲料、饮水加氟化钠１００ｍｇ／Ｌ或同时加碳酸钙３２００ｍｇ／ｋｇ饲料喂饲大鼠２个月，用流式细胞术检测肝细胞凋亡小体百分率，同时检测了谷胱苷肽过氧化物酶活性和脂质过氧化物含量。结果发现氟中毒组大鼠肝细胞凋亡小体百分率较对照组明显升高（Ｐ＝０．０１～０．０５），加钙可使这种改变更为明显（Ｐ＜０．０１）；而ＧＳＨ－ｐｘ活性则明显降低。结论氟化物的毒性可导致肝细胞凋亡，对机体抗氧化能力的降低可能起一定作用。

Objective To study the influence of fluoride and F+Ca on the hepatocyte apoptosis in rats.Methods The hepatocyte apoptosis was investigated by using flow cytometry and the GSH px activity was measured at the same time.Results The percentage of hepatocyte apoptosis bodies was higher in the group added fluoride ( P =0.01￣0.05) and much higher in the group added F+Ca ( P <0.01) than that of control;the GSH px activity was reduced in rats fed with F or F+Ca.Conclusions The hepatocyte apoptosis can be induced by fluoride and the change of GSH px may play an important role in the mechanism of hepatocyte apoptosis.