用过硫酸铵做氧化剂测定尿碘方法的研究

Measurement of urinary iodine by using ammonium persulfate as an oxidant

目的探索用过硫酸铵做为氧化剂测定尿碘方法的适宜因素。方法尿样经过硫酸铵消化后，又经过Ｓａｎｄｅｌ－Ｋｏｌｔｈｏｆ反应，最后用分光光度法测定尿碘含量。结果该方法的灵敏度为７μｇ／Ｌ；批间和批内变异系数均小于１０％；硫氰酸钾、维生素Ｃ和维生素Ｂ２对方法均无干扰；加入不同浓度标准后回收率在１００％～１０３％之间；与氯酸法有显著的线性相关关系（Ｐ＜０．０１）。结论该方法具有无爆、消化时无有害气体溢出、经济、方便等优点，特别适合发展中国家用于碘缺乏病监测。

Objective To study the optimal condition in the measurement of urinary iodine by using ammonium persulfate as an oxidant.Methods After being digested with ammonium persulfate,urinary specimen went through Sandell Kolthoff's Reaction.Then the concentration of iodine was measured by spectrometry method.Results The sensitivity of the method is 7μg/L;Both intra CV% and inter CV% are less than 10%;Potassium thiocynate,ascorbic acid and vitamin B 2 do not interfere with the measurement of urinary iodine;The recovery ranges between 100%￣103% in standard addition;This method also correlates with the chloric acid method,which was recommended by WHO,UNICEF and ICCIDD.Conclusions This method for urinary iodine is nonhazardous,nonexplosive,economic,and convenient,especially suitable for iodine deficiency disorders surveillance in developing countries.