血管内皮生长因子对人胚胎视网膜血管发生的调节作用

Regulation effect of vascular endothelial growth factor on angiogenesis in developmental human fetal retinas

目的研究血管内皮生长因子（ｖａｓｃｕｌａｒｅｎｄｏｔｈｅｌｉａｌｇｒｏｗｔｈｆａｃｔｏｒ，ＶＥＧＦ）对人胚胎视网膜血管发生的调节作用。方法收集５４例９～４０周龄胎儿眼球后壁标本，免疫组织化学染色，光镜观察。结果①ＶＥＧＦ在视网膜的表达呈波峰式分布，高峰在９～１３周及２６周左右。②节细胞层的梭形细胞（血管内皮细胞前体细胞）、血管内皮细胞呈增殖细胞核抗原（ｐｒｏｌｉｆｅｒａｔｉｏｎｃｅｌｎｕｃｌｅａｒａｎｔｉｇｅｎ，ＰＣＮＡ）免疫反应阳性，水平波动，高峰在９～１３周及２１周前后，此期间梭形细胞不断增殖、分化形成内皮细胞索，经改建形成视网膜内层血管，２６、３４周起见内核层内、外缘血管内皮细胞呈ＰＣＮＡ免疫反应阳性，并保持至足月。③视网膜ＶＥＧＦ表达量与梭形细胞、血管内皮细胞ＰＣＮＡ表达量呈显著正相关（ｒ＝０．７３６，Ｐ＜０．０１）。

Objective To investigate the spatial and temporal regulation effect of VEGF on human fetal retinal vascularization and angiogenesis. Methods The posterior segmental retinas from 54 human fetuses of the 9th week to the 40th week were studied by immunohistochemistry staining for the expressions of VEGF and PCNA. Results 1.The distribution of VEGF expression was spiking and the peaks were during the 9th～13th and around the 26th week.2.PCNA immunoreactivity was localized in spindle cells and vascular endothelial cells.The expression level was fluctuated during the developmental process.The peaks were during the 9th～13th and around the 21st week.In these periods,the spindle cells kept proliferating and differentiating,and remodelled subsequently to form the inner side retinal vessels.From the 26th or 34th week,the PCNA immunoreactivity is fully expressed in the vascular endothelial cells of the inner and outer margin of inner nuclear layer(INL) and kept to full terms.3.Significant positive correlation were shown between the content of VEGF in the retina and that of PCNA in spindle cells and vascular endothelial cells(r=0.736,P<0.01). Conclusion VEGF was positively involved in modulating human fetal retinal vascularization and angiogenesis.