8-氯腺苷诱导白血病细胞分化和凋亡

INDUCTION OF DIFFERENTIATION AND APOPTOSISIN LEUKEMIA CELLS BY 8-CHLOROADENOSINE

观察８－氯腺苷对人白血病细胞ＨＬ－６０和人Ｔ淋巴母细胞白血病细胞株（Ｍｏｌｔ－４）的分化和凋亡作用以及两种诱导效应的关系。流式细胞术－ＤＮＡ（ＦＣＭ－ＤＮＡ）测定显示８－氯腺苷使ＨＬ－６０和人Ｔ淋巴母细胞白血病细胞株Ｍｏｌｔ－４阻滞在Ｇ０／Ｇ１期。８－氯腺苷诱导人急性粒白血病细胞ＨＬ－６０分化，表现为硝基蓝四氮唑（ｎｉｔｒｏｂｌｕｅｔｅｔｒａｚｏｌｉｕｍ，ＮＢＴ）阳性细胞增多及出现形态学分化表型。通过形态学观察，ＦＣＭ－ＤＮＡ测定及琼脂糖电泳分析结果表明，８－氯腺苷可以诱导Ｍｏｌｔ－４细胞凋亡，且有时间、剂量依赖性，但未发现８－氯腺苷对ＨＬ－６０细胞有诱导凋亡的作用。

8-Chloroadenosine (8-Cl-Ado) is a new compound that is able to inhibit the growth of a wide variety of cancer cell types in vitro and in vivo. In this study we have found that differentiation of human acute myeloblastic leukemia(M2)cell line HL-60 can be induced by 8-Cl-Ado, FCM-DNA analysis showed that 8-Cl-Ado caused a block in G0/G1 phase of HL-60 and Molt-4 lymphoblast cell line. 8-Cl-Ado induced dose and time-dependent apoptosis in Molt-4 cells , but apoptosis was not observed in HL-60 cells treated with 8-Cl-Ado. These results suggest that induction of differentiation and apoptosis by 8-Cl-Ado is cell type specificity,and there is no causality between differentiation and apoptosis.