激活素A对Neuro-2a细胞电压门控钠电流的作用

Effects of activin A on voltage-gated Na^+ current of Neuro-2a cells

目的:研究激活素A(activinA)对Neuro-2a细胞电压门控钠电流(INa)的作用及其机制,为激活素A在神经系统疾病中的应用奠定基础。方法:培养小鼠源性Neuro-2a细胞,分为IgG对照组和抗激活素受体ⅡA(ActRⅡA)抗体染色组,免疫组织化学染色观察ActRⅡA在Neuro-2a细胞中是否表达;将Neuro-2a细胞随机分为正常对照组和激活素A组,采用全细胞膜片钳技术检测激活素A对Neuro-2a细胞INa的作用;采用RT-PCR检测激活素A对血管活性肠肽(VIP)及诱导型一氧化氮合酶(iNOS)mRNA表达的影响。结果:正常Neuro-2a细胞中可检测到ActRⅡA的表达;与正常对照组比较,激活素A明显增加Neuro-2a细胞INa(P<0.05);激活素A组Neuro-2a细胞VIPmRNA表达明显高于正常对照组(P<0.05),而iNOSmRNA表达低于正常对照组(P<0.05)。结论:激活素A具有增加Neuro-2a细胞电压门控Na+电流的作用,VIP和iNOS途径可能参与了激活素A调控神经细胞的功能。

Objective To study the effect of activin A on voltage-gated sodium current（INa）of Neuro-2acells and mechanism in order to lay a foundation for the application of activin A in nerve system diseases.Methods Neuro-2a cells were divided into IgG control group and anti-ActRⅡA antibody-stained group.Immunohistochemical staining was used to observe whether ActRⅡ A was expressed in Neuro-2acells.The Neuro-2acells were randomly divided into normal control group and activin A administration group.Whole cell patch clamp was used to analyze the effect of activin A on INaof Neuro-2acells.The expressions of vasoactive intestinal peptide （VIP）and inducible nitric oxide synthase （iNOS） mRNA were examined by RT-PCR in Neuro-2a cells treated with activin A. Results ActRⅡA expression was detectable in normal Neuro-2acells.Compared with control group,activin A increased INain Neuro-2acells significantly（P〈0.05）.The VIP mRNA expression in activin A group was higher than that in control group（P〈0.05）,while the expression of iNOS mRNA was lower than that in control group （P〈0.05）.Conclusion Activin A can increase INaof Neuro-2acells and the effect of activin A on regulating the neuron function may be mediated via VIP and iNOS pathways.